The Stickers and Spacers Framework for Describing Phase Behavior of Multivalent Intrinsically Disordered Proteins

内在无序蛋白质 生物系统 灵活性(工程) 细胞器 离解(化学) 聚合物 星团(航天器) 化学 计算机科学 生物物理学 纳米技术 化学物理 统计物理学 物理 材料科学 生物 数学 生物化学 物理化学 统计 有机化学 程序设计语言
作者
Jeong‐Mo Choi,Rohit V. Pappu
出处
期刊:Biophysical Journal [Elsevier BV]
卷期号:118 (3): 492a-492a 被引量:10
标识
DOI:10.1016/j.bpj.2019.11.2723
摘要

Accurate spatial and temporal organization of cellular components is essential for viability and functionality of a cell. Eukaryotic cells have invented membrane-bound organelles, but cells also employ membraneless organelles for rapid and efficient operations. In the last decade, it has been shown that liquid-liquid phase separation (LLPS) of biopolymers is the underlying principle behind formation, regulation, and dissociation of membraneless organelles in vivo. The LLPS drivers need to bear multivalency and flexibility, and intrinsically disordered proteins (IDPs) occasionally take the role. Although the amount of experimental data has been rapidly growing, however, our theoretical understanding is far from mature. We recently conceptualized the stickers and spacers framework, where IDP residues are designated into two groups: stickers, which drive chain-chain interactions, and spacers, which modulate the chain properties. Based on the framework, we developed an analytical mean-field model and applied it to the saturation concentration data of FUS family proteins to validate the utility of the model. The model predicts additive contributions from different types of sticker pairs, which explains why a dominant sticker pair type in one system can be insignificant in another system. We also demonstrate the results from graph-based simulations, which complement the analytical model. The simulation engine incorporates the concept of a cluster, which is a set of stickers that belong to the same polymer or to the polymers linked by sticker bonds. Intra-cluster interactions have different entropic contributions from inter-cluster ones, which the mean-field approach only considers. Moreover, three- and four-body interactions are implemented to test the effect of higher-order interactions. The mean-field predictions have been successfully reproduced, and it was shown that intra-cluster interactions are more crucial than multi-body interactions to explain the deviations of FUS variants from the mean-field prediction.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
多情凝荷完成签到 ,获得积分10
刚刚
补药完成签到,获得积分10
1秒前
leiiiiiiii完成签到,获得积分10
2秒前
浅色墨水完成签到,获得积分10
2秒前
YamDaamCaa应助wnche采纳,获得260
6秒前
华仔应助补药采纳,获得10
7秒前
MingQue完成签到,获得积分10
12秒前
jenningseastera完成签到,获得积分0
13秒前
17秒前
Ammr完成签到 ,获得积分10
17秒前
18秒前
充电宝应助liu采纳,获得10
18秒前
懵懂的雪糕完成签到 ,获得积分10
18秒前
ljx完成签到 ,获得积分10
20秒前
可爱的函函应助zifeimo采纳,获得10
21秒前
阳光沛柔发布了新的文献求助30
21秒前
赘婿应助踏山河采纳,获得10
24秒前
pluto应助ShengzhangLiu采纳,获得10
25秒前
26秒前
晴空万里完成签到,获得积分10
28秒前
Zsx关闭了Zsx文献求助
29秒前
OGB应助zhhr采纳,获得10
29秒前
30秒前
所所应助自觉竺采纳,获得10
30秒前
31秒前
科研通AI2S应助breath采纳,获得10
31秒前
怕孤单的山河完成签到 ,获得积分10
32秒前
35秒前
36秒前
qks完成签到 ,获得积分0
37秒前
37秒前
踏山河发布了新的文献求助10
37秒前
38秒前
38秒前
39秒前
Timber发布了新的文献求助30
40秒前
薄灯男孩发布了新的文献求助10
40秒前
刘明生发布了新的文献求助10
41秒前
搜集达人应助周诗琪采纳,获得10
41秒前
liu发布了新的文献求助10
45秒前
高分求助中
(禁止应助)【重要!!请各位详细阅读】【科研通的精品贴汇总】 10000
Semantics for Latin: An Introduction 1099
Biology of the Indian Stingless Bee: Tetragonula iridipennis Smith 1000
Robot-supported joining of reinforcement textiles with one-sided sewing heads 700
Thermal Quadrupoles: Solving the Heat Equation through Integral Transforms 500
SPSS for Windows Step by Step: A Simple Study Guide and Reference, 17.0 Update (10th Edition) 500
PBSM: Predictive Bi-Preference Stable Matching in Spatial Crowdsourcing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4126261
求助须知:如何正确求助?哪些是违规求助? 3663827
关于积分的说明 11593227
捐赠科研通 3363465
什么是DOI,文献DOI怎么找? 1848222
邀请新用户注册赠送积分活动 912232
科研通“疑难数据库(出版商)”最低求助积分说明 827935