泰乐菌素
色谱法
乙二醇二甲基丙烯酸酯
分子印迹聚合物
高效液相色谱法
固相萃取
化学
替米考星
螺旋霉素
分子印迹
甲基丙烯酸
选择性
单体
有机化学
红霉素
抗生素
聚合物
生物化学
催化作用
作者
Raquel Cañadas,Rosa María Goig Martínez,Gema Paniagua González,Pilar Fernández Hernando
出处
期刊:Polymer
[Elsevier]
日期:2022-05-01
卷期号:249: 124843-124843
被引量:8
标识
DOI:10.1016/j.polymer.2022.124843
摘要
A molecular imprinted membrane (MIM) was prepared for the selective binding of macrolide antibiotics by UV-initiated non-covalent imprinting approach. The membrane was modified by a UV-photographting technique in the presence of molecule templates of erythromycin (ERY) and spiramycin (SPI) with methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The nanofunctionalized MIM obtained was characterized by a morphological study using scanning electron microscopy (SEM), as well as by a study of its adsorption capacity by online solid phase extraction (SPE) procedure. Variables affecting the MIM-SPE method were optimized to maximize the extraction of macrolide antibiotics of interest and a high-performance liquid chromatography (HPLC) method was used for the analysis. Good linearity and precision were obtained for ERY and SPI, with average recoveries up to 86.14% and 34.73%, respectively, with a relative standard deviation (RSD) lower than 6%. In addition, selectivity was studied for other macrolides with similar structure to the templates, such as roxithromycin (ROX), josamycin (JOS), ivermectin (IVER) and tylosin (TYL). The proposed MIM-SPE-HPLC methodology was effectively applied to ERY and SPI determination in commercial doped semi-skimmed cow's milk samples.
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