试剂
聚丙烯酰胺凝胶电泳
核糖体蛋白
凝胶电泳
化学
单体
蛋白质凝胶电泳
聚丙烯酰胺
二聚体
色谱法
蛋白质亚单位
二维凝胶电泳
十二烷基硫酸钠
电泳
生物化学
高分子化学
有机化学
核糖体
聚合物
蛋白质组学
酶
核糖核酸
基因
作者
Andreas Urs Sommer,Robert R. Traut
标识
DOI:10.1073/pnas.71.10.3946
摘要
A diagonal polyacrylamide-dodecyl sulfate gel electrophoresis procedure is described. Its utility is related to the use of the reagent methyl-4-mercaptobutyrimidate as a protein-protein crosslinking reagent. Crosslinking with this reagent occurs through the formation of intermolecular disulfide bonds. Oxidized proteins are separated in one dimension by electrophoresis under non-reducing conditions and in the second dimension under reducing conditions. All proteins except those derived from crosslinked species fall on a diagonal. Methods are described for the identification of the separated monomeric components of crosslinked species. The technique has been applied to the 30S ribosomal subunit of Escherichia coli, and the new crosslinked dimer, S4-S13, has been identified.
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