染色质
计算生物学
清脆的
表观遗传学
转录因子
生物
单细胞分析
亚基因组mRNA
细胞
遗传学
基因
作者
Sarah E. Pierce,Jeffrey M. Granja,William J. Greenleaf
标识
DOI:10.1038/s41467-021-23213-w
摘要
Chromatin accessibility profiling can identify putative regulatory regions genome wide; however, pooled single-cell methods for assessing the effects of regulatory perturbations on accessibility are limited. Here, we report a modified droplet-based single-cell ATAC-seq protocol for perturbing and evaluating dynamic single-cell epigenetic states. This method (Spear-ATAC) enables simultaneous read-out of chromatin accessibility profiles and integrated sgRNA spacer sequences from thousands of individual cells at once. Spear-ATAC profiling of 104,592 cells representing 414 sgRNA knock-down populations reveals the temporal dynamics of epigenetic responses to regulatory perturbations in cancer cells and the associations between transcription factor binding profiles.
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