羟乙基淀粉
红细胞聚集
红细胞
蛋白质聚集
农业
聚合物
生物物理学
化学
右旋糖酐
血液粘度
桥接(联网)
生物化学
医学
生物
免疫学
内科学
纤维蛋白原
有机化学
血小板
计算机科学
计算机网络
作者
Sandra Henkelman,Gerhard Rakhorst,Henny C. van der Mei,Henk J. Busscher
出处
期刊:Clinical Hemorheology and Microcirculation
[IOS Press]
日期:2012-01-01
卷期号:52 (1): 27-35
被引量:9
摘要
Aggregation of human red blood cells (RBC) remains of biological and clinical interest. Replacement of plasma proteins by polymers to induce RBC aggregation may help to unravel the fundamentals of the aggregation process. Two theories exist to explain RBC aggregation mechanisms: a depletion and a bridging theory. RBC aggregation induced by hydroxyethyl starch (HES) increases with polymer size, which suggests that aggregation is induced via the bridging theory. In this study, the electrophoretic mobility (EPM) was measured to investigate RBC aggregation induced by 200 kDa HES polymers. Also, we evaluated if these polymers were useful for demonstrating aggregation differences between RBCs from healthy and type-1 diabetes mellitus (T1DM) subjects. Our results demonstrate that EPM values of RBCs in 200 kDa HES solutions were less negative than could be predicted by the viscosity of the suspension, supporting the bridging theory. Furthermore, aggregation analysis using the LORCA demonstrated that 200 kDa HES solution enhanced RBC aggregation of healthy and diabetic subjects similarly as standard 500 kDa dextran solutions.our data supports the bridging mechanism underlying 200 kDa HES induced RBC aggregation. In addition, both polymers are useful for demonstrating cellular induced aggregation differences between RBCs from healthy and T1DM subjects.
科研通智能强力驱动
Strongly Powered by AbleSci AI