亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

An aptamer and flower-shaped AuPtRh nanoenzyme-based colorimetric biosensor for the detection of profenofos

适体 检出限 辣根过氧化物酶 化学 生物传感器 石墨烯 线性范围 吸附 过氧化物酶 核化学 组合化学 纳米技术 色谱法 材料科学 有机化学 生物化学 分子生物学 生物
作者
Xin Tan,Wancui Xie,Qi Jia,Fangyuan Zhao,Wei Wu,Qingli Yang,Xiudan Hou
出处
期刊:Analyst [Royal Society of Chemistry]
卷期号:147 (18): 4105-4115 被引量:12
标识
DOI:10.1039/d2an00668e
摘要

In this work, a simple, sensitive and selective colorimetric method was established for the detection of profenofos. Firstly, novel flower-shaped AuPtRh trimetallic nanospheres were synthesized via a simple one-pot method, and had outstanding peroxidase catalytic activity. AuPtRh nanospheres with a great specific surface area were linked with an aptamer via Au-S and Pt-S bonds to specifically recognize profenofos. A graphene oxide grafted stainless-steel mesh (SSM-GO) was prepared to be a carrier and the aptamer-AuPtRh was nonspecifically adsorbed on the surface of SSM-GO, which was to be the capture probe for the detection of profenofos in real samples. They were characterized and confirmed by transmission electron microscopy, atomic force microscopy, etc. Through the investigation of the catalytic performance on the basis of the Michaelis equation, the Vmax of AuPtRh nanospheres was 22.27 × 10-8 M s-1, and Km was 0.6632 mM, which indicated that the affinity of AuPtRh nanospheres was relatively higher than that of horseradish peroxidase and Au NPs. In the presence of profenofos, the aptamer-AuPtRh would specifically combine with profenofos, which would further detach from SSM-GO. Then, it was introduced into the 3,3',5,5'-tetramethylbenzidine/H2O2 (TMB/H2O2) system to form blue oxTMB. The linear range of this colorimetric biosensor was 1-300 ng L-1 and the limit of detection was 0.725 ng L-1. It also had good recovery and anti-interference ability in real samples, which provided a new strategy for the rapid detection of pesticides.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
7秒前
12秒前
18秒前
Leah发布了新的文献求助10
21秒前
30秒前
Liufgui应助科研通管家采纳,获得10
30秒前
38秒前
科研通AI2S应助lalalatiancai采纳,获得10
43秒前
如沐春风发布了新的文献求助50
54秒前
www发布了新的文献求助10
54秒前
www完成签到,获得积分10
1分钟前
cyj完成签到 ,获得积分10
1分钟前
1分钟前
Orange应助如沐春风采纳,获得10
1分钟前
Jason发布了新的文献求助10
1分钟前
1分钟前
1分钟前
比比谁的速度快应助Jason采纳,获得10
1分钟前
lalalatiancai发布了新的文献求助10
1分钟前
超男完成签到 ,获得积分10
1分钟前
量子星尘发布了新的文献求助10
1分钟前
2分钟前
焦糖泡芙塔给焦糖泡芙塔的求助进行了留言
2分钟前
自信寻真发布了新的文献求助10
2分钟前
Benhnhk21完成签到,获得积分10
2分钟前
yx_cheng应助科研通管家采纳,获得10
2分钟前
2分钟前
lalalatiancai完成签到,获得积分10
2分钟前
2分钟前
量子星尘发布了新的文献求助10
3分钟前
3分钟前
3分钟前
4分钟前
Leah完成签到,获得积分10
4分钟前
Lucas应助科研通管家采纳,获得10
4分钟前
胡萝卜完成签到,获得积分10
4分钟前
量子星尘发布了新的文献求助10
4分钟前
Everything发布了新的文献求助10
5分钟前
蛋挞完成签到 ,获得积分10
5分钟前
Everything发布了新的文献求助10
5分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Social Research Methods (4th Edition) by Maggie Walter (2019) 2390
A new approach to the extrapolation of accelerated life test data 1000
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4008162
求助须知:如何正确求助?哪些是违规求助? 3547980
关于积分的说明 11298612
捐赠科研通 3282865
什么是DOI,文献DOI怎么找? 1810219
邀请新用户注册赠送积分活动 885957
科研通“疑难数据库(出版商)”最低求助积分说明 811188