First Report of Trichothecium roseum Causing Postharvest Fruit Rot on Purple Passion Fruit in China

HomePlant DiseaseVol. 106, No. 12First Report of Trichothecium roseum Causing Postharvest Fruit Rot on Purple Passion Fruit in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Trichothecium roseum Causing Postharvest Fruit Rot on Purple Passion Fruit in ChinaChenxu Li, Junlei Zhao, Jidong Wang, Xiangjing Wang, Wensheng Xiang, and Junwei ZhaoChenxu LiKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. China, Junlei ZhaoKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. China, Jidong WangKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science, Huzhou University, Huzhou, P.R. China, Xiangjing WangKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. China, Wensheng Xiang†Corresponding authors: W. Xiang; E-mail Address: [email protected], and J. Zhao; E-mail Address: [email protected]Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China, and Junwei Zhao†Corresponding authors: W. Xiang; E-mail Address: [email protected], and J. Zhao; E-mail Address: [email protected]https://orcid.org/0000-0003-1250-2756Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. ChinaAffiliationsAuthors and Affiliations Chenxu Li1 Junlei Zhao1 Jidong Wang1 2 Xiangjing Wang1 Wensheng Xiang1 3 † Junwei Zhao1 † 1Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, P.R. China 2Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science, Huzhou University, Huzhou, P.R. China 3State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Published Online:31 Oct 2022https://doi.org/10.1094/PDIS-01-22-0115-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePurple passion fruit (Passiflora edulis) is widely cultivated in many regions of southern China as an edible tropical fruit with excellent nutritional value and high economic value. In July 2021, postharvest fruit rot was observed on 20 to 25% of purple passion fruit in several fruit markets of Dehong City in Yunnan Province. Symptoms on infected fruits were irregular, pink-brown, soft, and water-soaked lesions, which enlarged and formed sunken patches with time as well as producing a small amount of white mycelium. To isolate the causal organism, five diseased fruits were collected from different fruit markets. A conidial mass from an individual sorus observed on an infected fruit was isolated and cultured on potato dextrose agar (PDA) supplemented with 50 μg ml−1 of streptomycin, and five fungal isolates were obtained. These isolates were morphologically similar and produced pale pink colonies on PDA after 7 days containing several conidiophores with abundant conidia. Mycelia were hyaline, 2 µm in diameter, and conidiophores were simple or branched (100 to 286 × 1.5 to 2.5 µm, n = 50). Conidia were pyriform, ovate, with papillary protuberances at one end. Almost all conidia were two celled and single septate (5.8 to 9.1 × 1.7 to 4.9 µm, n = 50). The morphology of the fungi resembled Trichothecium roseum as reported previously (Inácio et al. 2011). To further confirm the fungal species, isolate PASF4 was selected for molecular identification by amplifying and sequencing the ribosomal internal transcribed spacer (ITS) and large subunit (LSU) genes. Primers and PCR amplification were described by Fell et al. (2000). Results showed that both the ITS (GenBank accession OL336243) and LSU (OL336242) gene sequences had 100% similarity to T. roseum in NCBI database (MH856757 and MH868278). A maximum likelihood tree was constructed using MEGA 7 (Felsenstein 1981) based on concatenated sequences (ITS and LSU) of isolate PASF4 and reference strains. Phylogenetic analysis showed that isolate PASF4 belonged to the T. roseum clade. Based on morphological characteristics and phylogenetic analysis, isolate PASF4 was identified as T. roseum (Inácio et al. 2011). To confirm their pathogenicity, healthy purple passion fruits (cv. Tainong-1) were disinfected in 0.5% NaClO solution for 2 min and washed with sterile water. After wounding with a sterile needle, the fruits were inoculated by placing mycelium agar plugs on the wounds, and mock inoculation with mycelium-free PDA plugs served as a control. Five fruits were used in each treatment. All fruits were maintained in plastic boxes at 25°C. Disease symptoms appeared after inoculation for 4 to 7 days on all inoculated fruits, which were similar to those observed in fruit markets. No symptoms were observed on control fruits. The Trichothecium isolates were reisolated from symptomatic fruits, thus fulfilling Koch's postulates. Trichothecium roseum has been reported to cause fruit rot of tomato, apple, and orange in Pakistan (Hamid et al. 2014) and fruit rot of pepper in China (Lin et al. 2016). To our knowledge, this is the first report of T. roseum causing fruit rot on purple passion fruit worldwide, and these data will provide useful information for developing effective control strategies.The author(s) declare no conflict of interest.References:Fell, J. W., et al. 2000. Int. J. Syst. Evol. Microbiol. 50:1351. https://doi.org/10.1099/00207713-50-3-1351 Crossref, ISI, Google ScholarFelsenstein, J. 1981. J. Mol. Evol. 17:368. https://doi.org/10.1007/BF01734359 Crossref, ISI, Google ScholarHamid, M. I., et al. 2014. Plant Dis. 98:1271. https://doi.org/10.1094/PDIS-01-14-0051-PDN Link, ISI, Google ScholarInácio, C. A., et al. 2011. Plant Dis. 95:1318. https://doi.org/10.1094/PDIS-06-11-0464 Link, ISI, Google ScholarLin, S. H., et al. 2016. Plant Dis. 100:2164. Link, ISI, Google ScholarC. Li and J. Zhao contributed equally to this work.Funding: This work was supported in part by a grant from the Key Program of the National Natural Science Foundation of China (no. 32030090).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 12 December 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 15 Dec 2022 Published: 31 Oct 2022 First Look: 10 May 2022 Accepted: 5 May 2022 Page: 3212 Information© 2022 The American Phytopathological SocietyFundingNational Natural Science Foundation of ChinaGrant/Award Number: 32030090Keywordspassion fruitpostharvest fruit rotTrichothecium roseumThe author(s) declare no conflict of interest.PDF downloadCited byHigh-Quality Genome and Annotation Resource of Orange Pink Rot Pathogen Trichothecium roseum Strain YXFP-22015 Isolated from Hubei, ChinaLei Zhu, Zhaoyuan Wu, Wei Fang, and Yueying Wang5 June 2023 | Plant Disease, Vol. 107, No. 6