周质间隙
硫化地杆菌
生物电子学
纳米线
电子传输链
电子转移
地杆菌
生物物理学
细胞色素
化学
细胞外
纳米技术
材料科学
细菌
生物
生物化学
生物膜
光化学
生物传感器
酶
基因
大肠杆菌
遗传学
作者
Pilar C. Portela,Catharine Shipps,Cong Shen,Vishok Srikanth,Carlos A. Salgueiro,Nikhil S. Malvankar
标识
DOI:10.1038/s41467-024-46192-0
摘要
Extracellular electron transfer (EET) via microbial nanowires drives globally-important environmental processes and biotechnological applications for bioenergy, bioremediation, and bioelectronics. Due to highly-redundant and complex EET pathways, it is unclear how microbes wire electrons rapidly (>106 s-1) from the inner-membrane through outer-surface nanowires directly to an external environment despite a crowded periplasm and slow (<105 s-1) electron diffusion among periplasmic cytochromes. Here, we show that Geobacter sulfurreducens periplasmic cytochromes PpcABCDE inject electrons directly into OmcS nanowires by binding transiently with differing efficiencies, with the least-abundant cytochrome (PpcC) showing the highest efficiency. Remarkably, this defined nanowire-charging pathway is evolutionarily conserved in phylogenetically-diverse bacteria capable of EET. OmcS heme reduction potentials are within 200 mV of each other, with a midpoint 82 mV-higher than reported previously. This could explain efficient EET over micrometres at ultrafast (<200 fs) rates with negligible energy loss. Engineering this minimal nanowire-charging pathway may yield microbial chassis with improved performance.
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