高粱
生物
候选基因
基因
突变体
甜高粱
基因座(遗传学)
代谢途径
遗传学
基因组
植物
农学
作者
Li Mao,Qizhe Cai,Yinpei Liang,Yaofei Zhao,Hao Yaoshan,Yingying Qin,Xinrui Qiao,Yuanhuai Han,Hongying Li
摘要
Biomass yield is one of the important traits of sorghum, which is greatly affected by leaf morphology. In this study, a lobed-leaf mutant (sblob) was screened and identified, and its F2 inbred segregating line was constructed. Subsequently, MutMap and whole-genome sequencing were employed to identify the candidate gene (sblob1), the locus of which is Sobic.003G010300. Pfam and homologous analysis indicated that sblob1 encodes a Cytochrome P450 protein and plays a crucial role in the plant serotonin/melatonin biosynthesis pathway. Structural and functional changes in the sblob1 protein were elucidated. Hormone measurements revealed that sblob1 regulates both leaf morphology and sorghum biomass through regulation of the melatonin metabolic pathway. These findings provide valuable insights for further research and the enhancement of breeding programs, emphasizing the potential to optimize biomass yield in sorghum cultivation.
科研通智能强力驱动
Strongly Powered by AbleSci AI