化学
荧光
脂滴
生物物理学
荧光寿命成像显微镜
荧光显微镜
分辨率(逻辑)
融合
脂质双层
显微镜
尼罗河红
费斯特共振能量转移
纳米技术
膜
生物化学
光学
材料科学
物理
语言学
哲学
人工智能
计算机科学
生物
作者
Ning Xu,Qinglong Qiao,Xiangning Fang,Guangying Wang,Kai An,Wenchao Jiang,Jin Li,Zhaochao Xu
标识
DOI:10.1021/acs.analchem.4c00292
摘要
The varied functions of lipid droplets, which encompass the regulation of lipid and energy homeostasis, as well as their association with the occurrence of various metabolic diseases, are intricately linked to their dynamic properties. Super-resolution imaging techniques have emerged to decipher physiological processes and molecular mechanisms on the nanoscale. However, achieving long-term dynamic super-resolution imaging faces challenges due to the need for fluorescent probes with high photostability. This paper introduces LD-CF, a "buffering probe" for imaging lipid droplet dynamics using structured illumination microscopy (SIM). The polarity-sensitive LD-CF eliminates background fluorescence with a "cyan filter" strategy, enabling wash-free imaging of lipid droplets. In the fluorescent "off" state outside droplets, the probes act as a "buffering pool", replacing photobleached probes inside droplets and enabling photostable long-term SIM imaging. With this probe, three modes of lipid droplet fusion were observed, including the discovery of fusion from large to small lipid droplets. Fluorescence intensity tracking also revealed the direction of lipid transport during the lipid droplet fusion.
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