Preparation of Chromolaena odorata (Siam weed) loaded hydrogels and the effect of electrical potentials on the total flavonoids releasing behavior for wound healing application

ABSTRACTGelatin hydrogels containing the Chromolaena odorata (Siam Weed) crude extract were fabricated to study the morphology, the percentage of swelling, the total flavonoids released under a system without and with the external voltage stimulation, and the antibacterial properties. The percentage of pore area per cross-sectional area of the hydrogel was 23.56%. The hydrogels showed the extraordinarily high swelling percentage of 1,087.32 ± 16.16%. The total released flavonoids increased over time for both the systems without and with the external voltage stimulation. The total released flavonoid content from the systems with the external voltage stimulation was in larger quantities and more rapidly release comparing to the system in the absence of external voltage stimulation. The equivalent amount of the total released flavonoid tended to increase with the stimulating voltage of 0–0.3 V, but decreased when the excitation voltage was more than 0.3 V. Preferred amount of the total flavonoids released from hydrogels could be partly controlled by specific voltage stimulation. The fabricated hydrogel exhibits novel and great initial properties for wound healing materials and wound care application not only due to its extremely water absorption characteristic, but also perfectly in Gram-positive bacteria growth inhibition, which is ideal for the treatment of certain wounds with infection.KEYWORDS: Gelatin hydrogelChromolaena odorata (Siam weed)wound healing materialswound carebiomaterials Disclosure StatementNo potential conflict of interest was reported by the author(s).Author StatementPuripat Kantha: Project administration, Methodology, Investigation, Writing – review & editing. Sornchat Phiw-on: Investigation, Methodology. Onruthai Pinyakong: Writing – review & editing. Siriwan Teepoo: Writing – review & editing. Atipong Bootchanont: Writing – review & editing. Naris Barnthip: Conceptualization, Project administration, Resources, Methodology, Investigation, Supervision, Writing – original draft, Writing – review & editing.Data Availability StatementThe data that support the findings of this study are available from the corresponding author, [Barnthip, N.], upon reasonable request.Additional informationFundingThis work was funded by Rajamangala University of Technology Thanyaburi (RMUTT, Project code: 555287, Fiscal year: 2560) and partly supported by Thailand Toray Science Foundation, the 23rd Science & Technology Research Grant. The authors appreciate the support provided by the Division of Physics, Faculty of Science and Technology, RMUTT, and the Department of Microbiology, Faculty of Science, Chulalongkorn University, Thailand.