支气管肺泡灌洗
卵清蛋白
粘液
粘蛋白
炎症
脂多糖
化学
嗜酸性粒细胞
免疫学
分子生物学
免疫系统
药理学
医学
生物
内科学
生物化学
肺
哮喘
生态学
作者
Wenbing Zhi,Sheng-Nan Jiang,Zong-Ren Xu,Yuye An,Jing Chen,Ye Li,Yang Liu,Hong Zhang
出处
期刊:Fitoterapia
[Elsevier]
日期:2022-10-01
卷期号:162: 105278-105278
被引量:4
标识
DOI:10.1016/j.fitote.2022.105278
摘要
Asthma is a high-incidence disease in the world. Oxysophocarpine (OSC), a quinolizidine alkaloid displays various pharmacological functions including anti-inflammation, neuroprotective, anti-virus and antioxidant. Here, we established mice and cell asthmatic model to explore the effects of OSC for asthma treatment. Mice were sensitized and challenged with ovalbumin (OVA) and treated with OSC before challenge. Enzyme-linked immuno sorbent assay (ELISA), hematoxylin and eosin (H&E), periodic acid-schiff (PAS), tolonium chloride staining and immunohistochemical assay were performed. OSC treatment inhibited inflammatory cell infiltration and mucus secretion in the airway, reduced IgE level in mouse serum and decreased IL-4, IL-5 production in bronchoalveolar lavage fluid (BALF). OSC also reduced the spleen index to regulate immune function. Meanwhile, NCI-H292 cells were induced by lipopolysaccharide (LPS) to simulate airway epithelial injury. OSC pretreatment decreased the IL-6 and IL-8 cytokine levels, mucin 5 AC expression, and mucin 5 AC mRNA level in the cell model. Further, OSC suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), and activator protein 1 (AP-1, Fos and Jun). These findings revealed that OSC alleviated bronchial asthma associated with JNK/AP-1 signaling pathway.
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