精油
牛至
光滑假丝酵母
白色念珠菌
生物膜
最小抑制浓度
微生物学
百里香酚
白色体
两性霉素B
肉汤微量稀释
生物
传统医学
化学
食品科学
抗菌剂
抗真菌
细菌
医学
遗传学
作者
Mohammed Yassine Benziane,Mourad Bendahou,Fethi Benbelaïd,Abdelmounaim Khadir,Hanane Belhadef,Asma Benaissa,Saida Ouslimani,Fatma Mahdi,Alain Muselli
标识
DOI:10.1016/j.archoralbio.2022.105584
摘要
This study investigated the in vitro effect of Origanum glandulosum, Ammoides verticillata, and Saccocalyx satureioides essential oils against planktonic cells and biofilms formed by single and mixed species of Candida albicans and Candida glabrata oral isolates. The effect of the essential oils in combination with amphotericin B on planktonic cells was also studied.The antifungal susceptibility of planktonic cells was evaluated by disc diffusion and broth microdilution methods. Resazurin assay and scanning electron microscopy (SEM) were employed to determine the antibiofilm activity. The combinatory effect was evaluated by the checkerboard method. Essential oils were characterized by gas chromatography-mass spectrometry (GC-MS).The minimal inhibitory concentrations (MICs) and the minimum fungicidal concentrations (MFCs) of the studied essential oils were ranged between 250 and 2000 µg/mL. Biofilms were inhibited and eradicated by the essential oils at sub-inhibitory concentrations of 500 and 1000 µg/mL, respectively. SEM studies revealed a reduction in the preformed biofilm as a result of Origanum glandulosum essential oil treatment for single and mixed biofilms. Synergistic activity was found when Origanum glandulosum essential oil was combined with amphotericin B against Candida albicans. GC-MS analysis revealed that thymol was the major compound in Origanum glandulosum (38.36 %) and Ammoides verticillata (48.99 %) essential oils, while Saccocalyx satureioides essential oil was dominated by borneol (27.36 %).The studied essential oils showed significant antifungal and antibiofilm activities, which support their effectiveness as promising candidates for the management of oral Candida infections.
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