生物传感器
检出限
大肠杆菌
介电谱
微分脉冲伏安法
线性范围
循环伏安法
免疫分析
生物相容性
化学
色谱法
纳米复合材料
材料科学
电化学
纳米技术
核化学
组合化学
生物化学
抗体
电极
生物
有机化学
免疫学
物理化学
基因
作者
Chenyi Hu,Guanghua Wei,Fengfeng Zhu,Aiming Wu,Liuxuan Luo,Shuiyun Shen,Nengqin Jia,Junliang Zhang
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2023-05-08
卷期号:11 (20): 7894-7907
被引量:2
标识
DOI:10.1021/acssuschemeng.3c01241
摘要
Electrochemical immunosensors equipped with high specificity, superior analysis sensitivity, low cost, and convenient operations have been widely applied in the food industry, clinical medicine, biomonitor technique, and environmental protection. Because of the pressing requirements for detecting the pathogen Escherichia coli (E. coli) O157:H7 in various practical systems, an efficient and specific immunosensor was delicately designed based on the Pt@BSA nanocomposite with the surface conjugation of affinity-purified antibody molecules to achieve immune recognition. Amplified signal response toward probe molecules and favorable biocompatibility for bacteria attachment could be obtained at the assembled Pt@BSA biosensing interface. With the formation of immune complexes, specific binding of E. coli O157:H7 cells under a certain concentration range onto the antibody-immobilized interface resulted in electrochemical signal responses at different levels, which could be directly measured by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Herein, E. coli O157:H7 cells were quantitatively monitored by the DPV technique in a linear concentration range from 2.5 × 101 to 5.0 × 108 cfu mL–1 with a low detection limit of 9 cfu mL–1. This fabricated bacteria immunosensor indicated high specificity for E. coli determination, favorable selectivity among the interfering bacteria, excellent storage stability, satisfactory preparation reproducibility with a relative standard deviation (RSD) of 3.5%, and acceptable detection accuracy in practical food samples, providing a promising approach for ultrasensitive monitoring of hazardous substances and disease markers at an extremely low level in clinical diagnosis.
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