间质细胞
细胞生物学
脂肪组织
干细胞
生物
细胞外基质
体内
间充质干细胞
细胞分化
移植
细胞生长
细胞培养
癌症研究
遗传学
基因
内科学
医学
内分泌学
作者
Chao Wang,Tian Xie,Xiaoming Li,Xue Li,Changxue Xiao,Ping Liu,Feng Xu,Bo Zhang
标识
DOI:10.1016/j.mad.2024.111935
摘要
Adipose-derived stromal cells (ADSCs) are a promising stem cell sources for tissue engineering and cell-based therapy. However, long-term in vitro expansion of ADSCs impedes stemness maintenance, which is partly attributed to deprivation of their original microenvironment. Incompetent cells limit the therapeutic effects of ADSC-based clinical strategies. Therefore, reconstructing a more physiologically and physically relevant niche is an ideal strategy to address this issue and therefore facilitates the extensive application of ADSCs. Here, we transplanted separated ADSCs into local subcutaneous adipose tissues of nude mice as an in vivo cell culture model. We found that transplanted ADSCs maintained their primitive morphology and showed improved proliferation and delayed senescence compared to those of cells cultured in a incubator. Significantly increased expression of stemness-related markers and multilineage differentiation abilities were further observed in in vivo cultured ADSCs. Finally, sequencing revealed that genes whose expression differed between ADSCs obtained under in vivo and in vitro conditions were mainly located in the extracellular matrix and extracellular space and that these genes participate in regulating transcription and protein synthesis. Moreover, we found that an Egr1 signaling pathway might exert a crucial impact on controlling stemness properties. Our findings might collectively pave the way for ADSC-based applications.
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