生物
神经干细胞
祖细胞
神经球
祖细胞
干细胞
胚胎干细胞
细胞生物学
谱系(遗传)
胶质纤维酸性蛋白
人口
前体细胞
免疫学
成体干细胞
遗传学
细胞
基因
免疫组织化学
人口学
社会学
作者
Samantha Z. Yammine,Ian Burns,Jessica Gosio,Andrea Peluso,Daniel M. Merritt,Brendan Innes,Brenda L.K. Coles,Wen Yang,Gary D. Bader,Cindi M. Morshead,Derek van der Kooy
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2023-10-01
卷期号:32 (19-20): 606-621
标识
DOI:10.1089/scd.2023.0038
摘要
The mature brain contains an incredible number and diversity of cells that are produced and maintained by heterogeneous pools of neural stem cells (NSCs). Two distinct types of NSCs exist in the developing and adult mouse brain: Glial Fibrillary Acidic Protein (GFAP)-negative primitive (p)NSCs and downstream GFAP-positive definitive (d)NSCs. To better understand the embryonic functions of NSCs, we performed clonal lineage tracing within neurospheres grown from either pNSCs or dNSCs to enrich for their most immediate downstream neural progenitor cells (NPCs). These clonal progenitor lineage tracing data allowed us to construct a hierarchy of progenitor subtypes downstream of pNSCs and dNSCs that were then validated using single-cell transcriptomics. Further, we identify Nexn as required for neuronal specification from neuron/astrocyte progenitor cells downstream of rare pNSCs. Combined, these data provide single-cell resolution of NPC lineages downstream of rare pNSCs that likely would be missed from population-level analyses in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI