CX3CR1型
穿孔素
细胞毒性T细胞
CD8型
免疫学
流式细胞术
T细胞
颗粒酶
医学
免疫分型
系统性红斑狼疮
免疫系统
生物
内科学
趋化因子
趋化因子受体
疾病
生物化学
体外
作者
Qi Li,Zihang Yuan,Ayibaota Bahabayi,Zhonghui Zhang,Xingyue Zeng,Rui Kang,Qinzhu Xu,Ge Zhao,Pingzhang Wang,Chen Liu
标识
DOI:10.1016/j.intimp.2023.111231
摘要
This study investigated CX3CR1 expression in human peripheral blood T lymphocytes and their subsets, exploring changes in SLE patients and its diagnostic potential. Peripheral blood samples from 31 healthy controls and 50 SLE patients were collected. RNA-Seq data from SLE patient PBMCs were used to analyze CX3CR1 expression in T cells. Flow cytometry determined CX3CR1-expressing T lymphocyte subset proportions in SLE patients and healthy controls. Subset composition and presence of GZMB, GPR56, and perforin in CX3CR1+ T lymphocytes were analyzed. T cell-clinical indicator correlations were assessed. ROC curves explored CX3CR1′s diagnostic potential for SLE. CX3CR1+CD8+ T cells exhibited higher GPR56, perforin, and GZMB expression than other T cell subsets. The proportion of CX3CR1+ was higher in TEMRA and lower in Tn and TCM. PMA activation reduced CX3CR1+ T cell proportions. Both RNA-Seq and flow cytometry revealed elevated CX3CR1+ T cell proportions in SLE patients. Significantly lower perforin+ and GPR56+ proportions were observed in CX3CR1+CD8+ T cells in SLE patients. CX3CR1+ T cells correlated with clinical indicators. CX3CR1+ T cells display cytotoxic features, with heightened expression in CD8+ T cells, particularly in adult SLE patients. Increased CX3CR1 expression in SLE patient T cells suggests its potential as an adjunctive diagnostic marker for SLE.
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