化学
氨基脲
对接(动物)
枯草芽孢杆菌
抗菌活性
金黄色葡萄球菌
立体化学
细胞毒性
大肠杆菌
白色念珠菌
生物化学
细菌
有机化学
微生物学
体外
护理部
基因
生物
医学
遗传学
作者
Ghazaleh Zarrinzadeh,Mahmood Tajbakhsh,Rahman Hosseinzadeh,Mohammad A. Khalilzadeh,Masoumeh Hosseinzadeh
标识
DOI:10.1080/10406638.2021.2015405
摘要
Euparin (1) as a natural product was derivatized by maleic anhydride (2), semicarbazide (3), phenylacetic acid (4 and 5), and thiosemicarbazide (6) and their activities were evaluated in silico by molecular docking studies to investigate the binding affinities and interaction modes between the samples and N-myristoyltransferase enzyme. Among the tested samples (1–6), compounds 1–3 showed significant hydrophobic and hydrogen bonding interactions with the important residues at the active site of the receptor. Therefore, the biological properties of compounds 1–3 were investigated in this work. The antifungal activity of compounds 1–3 was tested against Candida albicans. Amongst them, euparin 1 with minimum inhibitory concentration (MIC) of 7.81 µg/mL and minimum fungicidal concentration (MFC) of 15.62 µg/mL exhibited the highest antifungal activity. Furthermore, antibacterial activities were studied against two Gram-positive (Staphylococcus aureus and Bacillus subtilis), and two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, the highest zone of growth on B. subtilis for compound 3 was 13.5 ± 0.7 mm, while for compound 2, the zone of growth inhibition against B. subtilis and S. aureus was 10.0 ± 1.4 and 9.5 ± 0.70 mm, respectively. Finally, the anticancer effects of 1– 3 were examined using MTT assay against MDA-MB-231 and A-549 cell lines. Compound 2 displayed cytotoxicity on MDA-MB-231 cell line with IC50 of 166 µg/mL.
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