放线菌门
淀粉酶
瘤胃
发酵
杆菌
淀粉
链霉菌
食品科学
生物
琼脂平板
细菌
微生物学
植物
酶
生物化学
遗传学
16S核糖体RNA
作者
Shanti Ratnakomala,Urip Perwitasari,- Yopi
出处
期刊:IOP conference series
[IOP Publishing]
日期:2020-02-01
卷期号:439 (1): 012019-012019
被引量:2
标识
DOI:10.1088/1755-1315/439/1/012019
摘要
Abstract Amylolytic actinobacteria were isolated from the rumen fluid of ruminants. Eighteen isolates actinobacteria were obtained and the generation of actinobacteria mutants using chemical agent Sodium Azide. The actinobacteria wild type and mutants were then screened for amylase activity using starch agar plate assay. Ten isolates showed the ability to produce amylase enzymes. Submerged fermentation (SmF) was used for microbial production of amylase. It was found that maximum amylase activity (2.53 U/mL) was produced by actinobacteria isolate R-9 M, and 2.43 U/mL was produced by isolate R-2 WT using pollard as a substrate in Submerged fermentation. This preliminary study could provide base information for the discovery of novel actinobacteria from natural resources such as rumen fluid for the production of amylase, which will be used for multipurpose.
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