Batch experiments were carried out to analyze the effect of temperature and fermentation time on lactic acid yield and optical characteristics during the non-sterilized fermentation of kitchen wastes. The results showed that the maximum total lactic acid production rate (0.59 g L-1 d-1) and yield (0.62 g per gram VS) were obtained at 35 ℃ after fermented for 120 h. Lactic acid (calculated as organic carbon) accounted for 78% of the total organic carbon in the fermentation liquid. The L-lactic acid was the predominant isomer form when the fermentation time was less than 120 h. At 35 ℃ and 55 ℃, L-lactic acid concentration decreased with the fermentation time, while at 45 ℃, it increased with the fermentation time and the ratio of L-lactic acid to total lactic acid reached 97% after fermented for 144 h. Fig 4, Tab 1, Ref 21