G蛋白偶联受体
逮捕
受体
HEK 293细胞
细胞生物学
化学
血管紧张素II
G蛋白
生物发光
生物物理学
生物
生物化学
作者
Dany Fillion,Dominic Devost,Terence E. Hébert
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 83-91
被引量:1
标识
DOI:10.1007/978-1-4939-9158-7_5
摘要
Initially identified as monomers, G protein-coupled receptors (GPCRs) can also form functional dimers that act as distinct signalling hubs for the integration of cellular signalling. We previously found that the angiotensin II (Ang II) type 1 receptor (AT1R) and the prostaglandin F2α (PGF2α) receptor (FP), both important in the control of smooth muscle contractility, form such a functional heterodimeric complex in HEK 293 and vascular smooth muscle cells (Goupil et al., J Biol Chem 290:3137-3148, 2015; Sleno et al., J Biol Chem 292:12139-12152, 2017). In addition to canonical G protein coupling, GPCRs recruit and engage β-arrestin-dependent pathways. Using BRET-based biosensors, we demonstrate how to assess recruitment of β-arrestin-1 and -2 to AT1R and the AT1R/FP dimer in response to Ang II. Surprisingly, β-arrestin-1 and -2 were recruited to the dimer, in response to PGF2α as well, even though FP alone cannot recruit either β-arrestin-1 and -2.
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