色谱法
化学
液相色谱-质谱法
质谱法
串联质谱法
作者
Dongxiao Sun,Lihua Dong,Panpan Guo,Xiaowei Shi,Jia Gao,Yi Ren,Xijuan Jiang,Wenxin Li,Chunying Wang,Qiao Wang
出处
期刊:Food Chemistry
[Elsevier]
日期:2013-05-01
卷期号:138 (1): 139-147
被引量:29
标识
DOI:10.1016/j.foodchem.2012.09.096
摘要
A sensitive liquid chromatography and tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous analysis of 15 flavonoids and 3 phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii. Separation was performed using a Diamonsil C(18) column, which was eluted with methanol (A) and 0.1‰ acetic acid (B). The gradient condition was as follows: 0-34 min, 20-34% A; 34-38 min, 34-95% A; maintained 95% A for the next 4 min. Analytes were detected using a hybrid quadrupole linear ion trap mass spectrometer that was equipped with an electrospray ionisation source in the negative ion and multiple-reaction monitoring modes. A full validation of the method was performed, including the linearity, precision, and accuracy, as well as limits of detection and quantification. The results indicated that the developed method was simple, sensitive and reliable. Furthermore, the method was successfully applied to differentiate 16 batches of Herba Lysimachiae and 21 batches of Herba Desmodii Styracifolii.
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