离解(化学)
子宫内膜异位症
核糖核酸
细胞
子宫内膜
生物
细胞生物学
基因
化学
癌症研究
病理
医学
内分泌学
遗传学
物理化学
作者
Yuliana Tan,Diane Luo,Suleyman Bozal,Paul Robson,Elise T. Courtois
标识
DOI:10.17504/protocols.io.bvy8n7zw
摘要
Tissue dissociation is critical step to ensure the quality of samples for single-cell RNA sequencing. To ensure recovery of most cells in the tissue without introducing stress response from the dissociation process, we adapted the dissociation method by Adam et al. (2017) and optimized for the dissociation of human endometrium and endometriosis tissue (both ectopic peritoneal and ovary lesions), targeting high quality viable cells (above 70%) with high transcript and gene counts per cell.
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