嗜肺军团菌
检出限
生物传感器
军团菌
污染
微生物学
材料科学
细菌
生物
化学
纳米技术
色谱法
生态学
遗传学
作者
Mariacristina Gagliardi,Matteo Agostini,Francesco Lunardelli,Leonardo Lamanna,Alessio Miranda,Agostino Bazzichi,Antonella Giuliana Luminare,Fabrizio Cervelli,Francesca Gambineri,Michele Totaro,Michele Lai,Giuseppantonio Maisetta,Giovanna Batoni,Mauro Pistello,Marco Cecchini
标识
DOI:10.1016/j.snb.2023.133299
摘要
Surface acoustic wave (SAW) -based immuno-biosensors are used for several applications, thanks to their versatility and faster response than conventional analytical methods. SAW immuno-biosensors can be usefully applied to promptly detect bacteria and prevent bacterial infections that can lead to severe diseases. Here, we present a SAW immuno-biosensor to detect Legionella pneumophila in water. Our device, working at ultra-high frequency (740 MHz), is functionalized with an anti-L. pneumophila antibody to maximize the specificity. We report the characteristic curve of the sensor, calculated measuring bacterial samples at known densities, and its related parameters. We also measure L. pneumophila samples contaminated with different Gram-positive and Gram-negative bacterial species (Escherichia coli and Enterococcus faecium) and samples diluted in mains waters. The proposed device is able to detect L. pneumophila in the range from 1·106 to 1·108 CFU/mL, with a limit of blank of 1.22·106 CFU/mL and a limit of detection of 2.01·106 CFU/mL. The nonspecific signal due to contaminant bacteria is very limited and measurements of L. pneumophila are not affected by contamination. We obtain a good detection also in mains water, representing a realistic matrix for L. pneumophila. Our results are encouraging and pave the way to the use of fast, easy-to-use, reliable and precise sensors to prevent bacterial infections in human activities.
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