牙周膜干细胞
运行x2
牙周纤维
医学
干细胞
牙槽
CD90型
破骨细胞
碱性磷酸酶
牙周炎
间充质干细胞
成骨细胞
化学
病理
细胞生物学
内科学
牙科
体外
川地34
生物
生物化学
受体
酶
作者
Shuang Lai,Cong Liu,Liyuan Fan,Li Deng,Yiling Yang,Chang Liu,Yushu Zhu,Xinlun Li,Xiao Li,Yandong Mu
出处
期刊:Oral Diseases
[Wiley]
日期:2022-11-02
卷期号:29 (8): 3503-3513
被引量:2
摘要
A lack of relevant research on Lycium barbarum polysaccharide-glycoprotein (LBP) application in oral diseases. Here, we focused on the effect of LBP on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and periodontitis bone loss.Human periodontal ligament stem cells (hPDLSCs) were isolated and identified by flow cytometry. Alkaline phosphatase (ALP) activity, Alizarin Red staining, and combined qPCR and Western blot analyses were performed to elucidate the effects of LBP on the osteogenic potential of hPDLSCs. In vivo experiments were performed with the treatment of LBP in rat periodontal model. MicroCT scanning and histological analysis were conducted to evaluate osteogenesis in situ.Human periodontal ligament stem cells (hPDLSCs) were successfully isolated and identified with CD90, CD29, and CD45. LBP enhanced hPDLSCs proliferation and migration and promoted RUNX2, ALP, Collagen I, and Osteocalcin expression through activating the ERK1/2 signaling pathway in vitro. The inflammatory factors, including interleukin 6 (IL-6) and interleukin 8 (IL-8) were reduced after LBP treatment. Alveolar bone resorption was significantly decreased in the LBP-treated groups in vivo, and osteoclast was markedly decreased by LBP application.LBP promoted hPDLSC osteogenesis by targeting the ERK1/2 signaling pathway and reverse bone loss by reducing inflammation. These findings provided latent hope for LBP application in periodontal therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI