Jurkat细胞
T细胞受体
细胞毒性T细胞
生物
转导(生物物理学)
T细胞
分子生物学
人类白细胞抗原
主要组织相容性复合体
克隆(Java方法)
细胞培养
BETA(编程语言)
细胞生物学
抗原
基因
免疫学
遗传学
免疫系统
生物化学
体外
程序设计语言
计算机科学
作者
Anna Calogero,Geesiena Hospers,K. M. Krüse,Schrier Pi,Nanno Mulder,Erik Hooijberg,de Louis Leij
出处
期刊:PubMed
日期:2000-08-06
卷期号:20 (3A): 1793-9
被引量:15
摘要
The T cell receptor (TCR) is an heterodimeric protein on the cell membrane of cytotoxic T cells (CTLs). In CTLs TCRs mediate the recognition of target cells through interaction with specific, MHC class I presented peptides.As a model system to show proof of principle we chose the Jurkat/MA cell line and the HLA-A2.1 binding MAGE-3 derived peptide 271-279, as target specificity.We show that this cell line can be successfully transduced with the dicistronic retroviral vector (LZRS) containing cDNAs encoding for the complete alpha and beta chains of the selected TCR. Following retroviral transduction, Jurkat/MA cells do express the anti-MAGE-3 TCR on their membrane. The transduced TCR is functional as travoductants are successfully triggered, upon stimulation with T2 cells or MAGE-3+ melanoma cells loaded with the MAGE-3 peptide.We conclude that TCR gene transfer is possible and it represents a powerful therapeutic tool for the genetical modification of T calls of patients sullering from cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI