化学
MAPK/ERK通路
激酶
免疫印迹
乙醇
磷酸化
体外
信号转导
脂多糖
细胞凋亡
立体化学
乙酸乙酯
生物化学
免疫学
生物
基因
作者
Yan Sun,Peng Jiang,Yi-Kai Jiang,Juan Pan,Jia-Tong Wu,Xiao-Mao Li,Wei Guan,Xin-Yu Min,Yu-Xuan Wang,Hai-Xue Kuang,Yan Liu,Bing-You Yang
标识
DOI:10.1016/j.bioorg.2023.106447
摘要
Fifteen new chromones, sadivamones A-E (1-5), cimifugin monoacetate (6), sadivamones F-N (7-15), together with fifteen known chromones (16-30), were isolated from the ethyl acetate portions of 70% ethanol extract of Saposhnikovia divaricata (Turcz.) Schischk roots. The structures of the isolates were determined using 1D/2D NMR data and electron circular dichroism (ECD) calculations. Meanwhile, LPS induced RAW264.7 inflammatory cell model was used to determine the potential anti-inflammatory activity of all the isolated compounds in vitro. The results showed that compounds 2, 8, 12-13, 18, 20-22, 24, and 27 significantly inhibited the production of lipopolysaccharide (LPS)-induced NO in macrophages. To determine the signaling pathways involved in the suppression of NO production by compounds 8, 12 and 13, we investigated ERK and c-Jun N-terminal protein kinase (JNK) expression by western blot analysis. Further mechanistic studies demonstrated that compounds 12 and 13 inhibited the phosphorylation of ERK and the activation of ERK and JNK signaling in RAW264.7 cells via MAPK signaling pathways. Taken together, compounds 12 and 13 may be valuable candidates for the treatment of inflammatory diseases.
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