What does the PERV copy number tell us?

XenotransplantationVolume 29, Issue 2 e12732 LETTER TO THE EDITOR What does the PERV copy number tell us? Joachim Denner, Corresponding Author Joachim Denner [email protected] orcid.org/0000-0003-3244-6085 Institute of Virology, Free University Berlin, Berlin, Germany Correspondence Joachim Denner, Institute of Virology, Free University Berlin, Berlin, Germany. Email: [email protected]Search for more papers by this author Joachim Denner, Corresponding Author Joachim Denner [email protected] orcid.org/0000-0003-3244-6085 Institute of Virology, Free University Berlin, Berlin, Germany Correspondence Joachim Denner, Institute of Virology, Free University Berlin, Berlin, Germany. Email: [email protected]Search for more papers by this author First published: 02 February 2022 https://doi.org/10.1111/xen.12732Citations: 1Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. REFERENCES 1Denner J. How active are porcine endogenous retroviruses (PERVs)?. Viruses. 2016; 8:215. https://doi.org/10.3390/v8080215. 2Wang M, Yang J, Gai Z, et al. Comparison between digital PCR and real-time PCR in detection of Salmonella typhimurium in milk. Int J Food Microbiol. 2017; 266: 251- 256. 3Tang H, Cai Q, Li H, Hu P. 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Identification of a novel type C porcine endogenous retrovirus: evidence that copy number of endogenous retroviruses increases during host inbreeding. J Gen Virol. 2001; 82: 1829- 1834. 17Denner J, Schuurman HJ. High prevalence of recombinant porcine endogenous retroviruses (PERV-A/Cs) in minipigs: a review on origin and presence. Viruses. 2021; 13(9): 1869. 18Subramanian RP, Wildschutte JH, Russo C, Coffin JM. Identification, characterization, and comparative genomic distribution of the HERV-K (HML-2) group of human endogenous retroviruses. Retrovirology. 2011; 8: 90. 19Contreras-Galindo R, Kaplan MH, He S, et al. HIV infection reveals widespread expansion of novel centromeric human endogenous retroviruses. Genome Res. 2013; 23(9): 1505- 1513. 20Groenen MA, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF. Analyses of pig genomes provide insight into porcine demography and evolution. Nature. 2012; 491: 393- 398. 21Scobie L, Taylor S, Wood JC, et al. Absence of replication-competent human-tropic porcine endogenous retroviruses in the germ line DNA of inbred miniature swine. J Virol. 2004; 78(5): 2502- 2509. 22Denner J, Scobie L. Are there better assays to evaluate the risk of transmission of porcine endogenous retroviruses (PERVs) to human cells?. Xenotransplantation. 2019; 26: 4. 23Godehardt AW, Petkov S, Gulich B, Fischer N, Niemann H, Tönjes RR. Comparative gene expression profiling of pig-derived iPSC-like cells: effects of induced pluripotency on expression of porcine endogenous retrovirus (PERV). Xenotransplantation. 2018; 25(4):e12429. 24Martin U, Kiessig V, Blusch JH, et al. Expression of pig endogenous retrovirus by primary porcine endothelial cells and infection of human cells. Lancet. 1998; 352(9129): 692- 694. 25Dieckhoff B, Kessler B, Jobst D, et al. Distribution and expression of porcine endogenous retroviruses in multi-transgenic pigs generated for xenotransplantation. Xenotransplantation. 2009; 16(2): 64- 73. 26Wilson CA, Wong S, Muller J, Davidson CE, Rose TM, Burd P. Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells. J Virol. 1998; 72(4): 3082- 3087. 27Tacke SJ, Specke V, Denner J. Differences in release and determination of subtype of porcine endogenous retroviruses produced by stimulated normal pig blood cells. Intervirology. 2003; 46(1): 17- 24. https://doi.org/10.1159/000068120. 28Denner J. Why was PERV not transmitted during preclinical and clinical xenotransplantation trials and after inoculation of animals?. Retrovirology. 2018; 15(1): 28. https://doi.org/10.1186/s12977-018-0411-8. Citing Literature Volume29, Issue2March/April 2022e12732 ReferencesRelatedInformation