适体
费斯特共振能量转移
核糖核酸
生物膜
铜绿假单胞菌
化学
纳米技术
生物
生物物理学
计算生物学
微生物学
细胞生物学
基因
细菌
生物化学
荧光
分子生物学
材料科学
遗传学
物理
量子力学
作者
Ya Gao,Yurui Xu,Yanyan Li,Kerong Chen,Xiaotong Wu,Yuhang Liu,Xuli Feng,Desheng Kong,Xinghai Ning
出处
期刊:Nano Letters
[American Chemical Society]
日期:2022-01-07
卷期号:22 (2): 716-725
被引量:5
标识
DOI:10.1021/acs.nanolett.1c03970
摘要
An effective method to identify c-di-GMP may significantly facilitate the exploration of its signaling pathways and bacterial pathogenesis. Herein, we have developed the first conjugated polymer-amplified RNA aptamer NanoKit with a unique core-shell-shell architecture, which combines the advantages of high selectivity of RNA aptamers and high sensitivity of strong fluorescence resonance energy transfer (FRET) effect, for precisely detecting c-di-GMP. We identified that NanoKit could selectively detect c-di-GMP with a low detection limit of 50 pM. Importantly, NanoKit could identify bacterial species and physiological states, such as planktonic, biofilm, and even antibiotic-resistance, on the basis of their different c-di-GMP expression patterns. Particularly, NanoKit could distinguish bacterial infection and inflammation and identify Pseudomonas aeruginosa associated pneumonia and sepsis, thereby guiding treatment choice and monitoring antibiotic effects. Therefore, NanoKit provides a promising strategy to rapidly identify c-di-GMP and its associated diseases and may benefit for pathophoresis management.
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