激光捕获显微切割
显微解剖
乳腺癌
病理
蛋白质组学
生物
癌细胞
生物标志物发现
基质
电池类型
人口
生物标志物
癌症
细胞
免疫组织化学
医学
基因表达
基因
环境卫生
生物化学
遗传学
作者
René B. H. Braakman,Theo M. Luider,John W.M. Martens,John A. Foekens,Arzu Umar
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 143-154
被引量:17
标识
DOI:10.1007/978-1-61779-163-5_11
摘要
Breast cancer tissues are characterized by cellular heterogeneity, representing a mixture of, e.g., healthy epithelial ducts, invasive or in situ tumor cells, surrounding stroma, infiltrating immune cells, blood vessels, and capillaries. As a consequence, protein extracts from whole tissue lysates also represent a variety of cell types present in the tissues under examination. This, however, seriously hampers the analysis of tumor cell-specific signals, which is of interest when performing biomarker discovery-type of studies. Therefore, laser capture microdissection is a perfect tool to isolate a relatively pure population of cells of interest, such as tumor cells. In this chapter, we describe the use of the PALM MicroBeam system for laser microdissection and pressure catapulting. Protocols are provided for sectioning, staining, microdissection, sample preparation, and mass spectrometric analysis of snap frozen breast cancer tissue.
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