癌症
长非编码RNA
癌症研究
转移
体外
比例危险模型
生物
危险系数
癌细胞
实时聚合酶链反应
微阵列
生物标志物
下调和上调
医学
内科学
基因表达
基因
生物化学
置信区间
作者
Fa Chen,Lingjun Yan,Jing Wang,Lisong Lin,Yu Qiu,Zhijian Hu,Junfeng Wu,Xiaodan Bao,Li Lin,Rui Wang,Lin Cai,Fengqiong Liu,Baochang He
出处
期刊:Head & neck
[Wiley]
日期:2019-04-10
卷期号:41 (9): 2883-2891
被引量:6
摘要
Abstract Background The aim of the present study was to assess the long noncoding RNA (lncRNA) expression profile of oral cancer and clarify the biological functions and clinical value of a novel lncRNA ENST00000470447.1. Method Microarray assay was used to explore lncRNA expression profiles in three paired oral cancer and adjacent normal tissue samples. The expression of targeted lncRNA (ENST00000545372.1, ENST00000470447.1, and ENST00000412353.1) was validated by quantitative real‐time polymerase chain reaction. Then we evaluated the biological and clinical roles of ENST00000470447.1 in oral cancer by in vitro assays and multivariable Cox regression analysis. Results LncRNAs were aberrantly expressed in oral cancer tissues. The expression levels of ENST00000470447.1 and ENST00000412353.1 in oral cancer tissues were significantly lower than those in matched adjacent noncancerous tissues ( P < .001). in vitro assays indicated that overexpression of ENST00000470447.1 significantly inhibited the proliferation, migration, and invasion ability of Tca‐8113 cells, whereas promoted the apoptosis of Tca‐8113 cells. Furthermore, ENST00000470447.1 expression was significantly correlated with tumor differentiation ( P = .030). Cox regression analyses demonstrated that high ENST00000470447.1 expression was associated with better disease‐free survival for patients with oral cancer (hazard ratio: 0.25, 95% CI: 0.06‐0.95; P = .041). Conclusion Our findings suggest that ENST00000470447.1 can be served as a potential novel marker for recurrence and metastasis prediction of patients with oral cancer, which may provide a potential therapeutic target for oral cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI