葡萄糖酸
化学
催化作用
沸石咪唑盐骨架
检出限
共沉淀
葡萄糖氧化酶
吸光度
降级(电信)
咪唑酯
还原剂
线性范围
纳米结构
化学工程
组合化学
核化学
材料科学
生物传感器
纳米技术
无机化学
色谱法
有机化学
金属有机骨架
吸附
工程类
电信
生物化学
计算机科学
作者
Zhenghong Zhao,Tianran Lin,Wenren Liu,Li Hou,Fanggui Ye,Shulin Zhao
标识
DOI:10.1016/j.saa.2019.04.061
摘要
Zeolitic imidazolate framework-8 (ZIF-8) has become one of the most typical examples of nanostructures for multi-enzyme immobilization due to its economical, mild and easy synthesis process. However, ZIF-8 nanocrystals are easily decomposed under acidic conditions. To solve this problem, the Fe-polydopamine (Fe-PDA) was bonded with ZIF-8 surface to form [email protected] hybrid shell with good stability. Based on this, we developed glucose [email protected]@Fe-PDA ([email protected]@Fe-PDA) integrated nanozymes (INAzymes) with cascade reactions via a mild and environmentally friendly method. In order to synthesize the INAzymes, GOx was first embedded in ZIF-8 by coprecipitation ([email protected]), and then [email protected] was bonded with Fe-PDA, which acted as a peroxidase mimic. The [email protected] hybrid shell protected the INAzymes nanostructure from degradation under acidic conditions, which results in good chemical stability of the [email protected]@Fe-PDA. In the INAzymes system, glucose is converted to gluconic acid by GOx in the presence of oxygen to produce H2O2 as an intermediate. The H2O2 reacts rapidly with Fe-PDA to generate OH, which oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB). The UV absorbance of oxidized TMB is directly proportional to the glucose concentration, and has a good linear relationship in the range of 5.0–100.0 μM glucose with detection limit of 1.1 μM. The INAzymes system has been successfully applied to rapid colorimetric detection of blood glucose levels. The INAzymes system exhibits high catalytic activity, excellent sensitivity, and enhanced chemical stability, playing great promise in clinical diagnosis and biosensing.
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