Cultivation and Detection of Sulfate Reducing Bacteria (SRB) in Sea Water

Sulfate-reducing bacteria (SRB) represent a class of anaerobic microorganisms that conduct dissimulatory sulfate reduction to obtain energy. The present study aimed to detect and control SRB activities using a very rapid detectable culture medium and reduction of potential economic loss in the petroleum sector. This study is an attempt to isolate SRB from sea water by rapid and sensitive culture media and to control their effect using eight commercial biocides (Aldehydes and quaternaries). The present work studies the effect of composition of four recommended culture media (Postgate medium B, Starkey, Baar's and API media), besides, the presence of metal coupons in these media to enhance the growth of sessile SRB. Furthermore, the present study evaluates the efficiency of filtration of these culture media on the growth of SRB. The results revealed that modified Postgate medium B was the recommended medium for SRB growth. In addition, the results showed that rapid and abundant growth of SRB when the metal coupons were immersed in the culture media which were deficient in iron. The unfiltered culture media improved the SRB growth. The growth of SRB was depressed by 15 ppm of the commercial quaternaries rather than 20 ppm of the aldehydes. (E. A. Ghazy, M.G. Mahmoud, M. S. Asker, M. N. Mahmoud, M. M. Abo Elsoud and M. E. Abdel Samie. Cultivation and detection of sulfate reducing bacteria (SRB) in sea water. Journal of American Science 2011; 7(2):604-608). (ISSN: 1545-1003). http://www.americanscience.org 0.09 % (C), 0.07 % (Si), 0.37 % (Mn), 0.017 % (S), 0.028 % (P), 0.005 %(Al), 0.015 %(Ni), 0.11 %(Cr), 0.004 % (Mo), 0.006 % (Cu), and 0.007 % (V).The electrodes were polished with emery papers 200.400, 600, grade for fine polishing .They were washed with distilled water then degreased with acetone and finally dried till use. 2.4. Pipe line description by ESHPETCO Length 7 Km (above the ground surface); diameter 18, construction date since 1982; fluid was crude petroleum oil with water content 0.05 % Vol. sulfur content 4.5 % wt, pH value was 6-6, 5 and temperature 20-30