Fermentation soybean peptide liquid was produced by Aspergillus oryzae A-9005 with fermented soybean meal.The effects of soybean meal concentration,carbon nitrogen ratio(C/N),and fermentation temperature and time on fermentation were studied.The optimal fermentation conditions were obtained by orthogonal experiments as follows: soybean meal content 3%,C/N 14,fermentation temperature 30℃ and fermentation time 46h.Under these conditions,the conversion rate of soybean peptide in the fermentation liquor reached 55.6%.The percentage of molecular weight of soybean peptide was 43.28%,which distributed from 204u to 1335u.