寻常变形杆菌
银纳米粒子
傅里叶变换红外光谱
核化学
粘质沙雷氏菌
化学
扫描电子显微镜
高分辨率透射电子显微镜
透射电子显微镜
最小抑制浓度
吖啶
抗菌活性
纳米颗粒
细菌
微生物学
材料科学
纳米技术
抗菌剂
大肠杆菌
生物
生物化学
有机化学
化学工程
复合材料
工程类
基因
植物
遗传学
作者
Nelsonjoseph Lawrance,Vishnupriya Benaltraja,Amsaveni Ramasamy,Devaraj Bharathi,T.K.Shabeer and A.Subramani,Rehna Parameswaran
标识
DOI:10.1016/j.bcab.2021.102222
摘要
This study explains the mycosynthesis of silver nanoparticles (AgNPs) using Acremonium borodinense by a green route. The synthesized AgNPs were characterized by using UV–vis Spectroscopy, X-ray diffraction spectroscopy (XRD), field emission scanning electron microscopy (FE-SEM), high-resolution transmission electron microscopy (HRTEM) and fourier transform infra-red (FTIR) spectroscopy. XRD technique revealed that the face centered cubic crystalline structure of AgNPs. FE-SEM and TEM images showed that the synthesized AgNPs having spherical shaped nanostructure with an average size of 0.19 nm. The functional groups present in the synthesized AgNPs were studied by FTIR. Mycosynthesized AgNPs were stable up to 3 months without change in their properties. Pathogenic bacteria proliferation was reduced by modest dosages of AgNPs. The minimum inhibitory concentration and minimum bacterial concentration of AgNPs were determined against both gram-positive and gram-negative bacterial pathogens. Such as Staphylococcus aureus, Proteus vulgaris, Serratia marcescens, Klebsiella pneumonia. Highest Value of MIC (90 μg/mL) 0.098 was found against Proteus vulgaris. Antibacterial activity of AgNPs was investigated at three different concentrations based on MIC (30, 60, and 90 g/mL). The impact of AgNPs on Red Blood Cells from the ‘O' positive blood group was investigated, and it was shown that even at the highest concentrations, AgNPs does not induce higher hemolysis in the cells. Based on these finding, we strongly suggested that the Acremonium borodinense mediated synthesized AgNPs can be used as a potent bio-medical agent.
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