类有机物
3D生物打印
脊髓
神经干细胞
细胞生物学
明胶
细胞分化
干细胞
生物
生物医学工程
解剖
组织工程
神经科学
医学
基因
生物化学
作者
Yilin Han,Marianne King,Evgenii Tikhomirov,Povilas Barasa,Cleide Dos Santos Souza,Jonas Lindh,Daiva Baltriukienė,Laura Ferraiuolo,Mimoun Azzouz,Maurizio R. Gullo,Elena N. Kozlova
摘要
Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation.
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