硝基还原酶
化学
荧光
缺氧(环境)
体内
选择性
生物化学
生物物理学
炎症
细胞
检出限
癌细胞
细胞生物学
酶
生物
癌症
氧气
色谱法
免疫学
量子力学
遗传学
有机化学
催化作用
生物技术
物理
作者
Wei Wang,Jiexuan Cai,Nai-Kei Wong,Meijing Hong,Jianbin Deng,Long Jin,Yang Ran,Yi Zhang,Yaqi Zhou,Bai‐Ou Guan
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:147 (7): 1449-1456
被引量:5
摘要
Detecting nitroreductase (NTR) activity in hypoxic cells and tissues in situ represents an important step toward accurate delineation of hypoxic disease loci. However, it remains challenging to develop fluorescent probes with the necessary attributes of selectivity, sensitivity, precise targeting and aqueous solubility. Herein, two kinds of fluorescent probes (NNP and cRGD-NNP) built on a 2-nitroimidazole sensing platform were synthesized for the detection of NTR activity in cell and in vivo models of hypoxia. In the presence of NADH, NNP displayed high selectivity for NTR, a strong fluorescence enhancement (108 fold), and a low detection limit (3.6 ng mL-1). Benefiting from the hydrophilic structure and tumor-targeting properties of the cRGD cyclopeptide group, the probe cRGD-NNP efficiently detected NTR activity in MCF cancer cells under hypoxia. In addition, the liposome-encapsulated probe was successfully applied to visualize NTR during liver inflammation in mice.
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