污渍
乙酰化
磷酸化
泛素
翻译后修饰
DNA损伤
细胞生物学
生物
计算生物学
DNA
生物化学
基因
酶
作者
Anna Estevan Barber,David W. Meek
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 7-18
标识
DOI:10.1007/978-1-0716-1217-0_2
摘要
The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.
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