子宫内膜异位症
小RNA
接收机工作特性
生物标志物
肿瘤科
逻辑回归
医学
微阵列
内科学
曲线下面积
微阵列分析技术
实时聚合酶链反应
曲线下面积
诊断生物标志物
生物信息学
生物
诊断准确性
基因
基因表达
遗传学
药代动力学
作者
Emine Çoşar,Ramanaiah Mamillapalli,Gülçı̇n Şahin Ersoy,SihYun Cho,Benjamin J. Seifer,Hugh S. Taylor
标识
DOI:10.1016/j.fertnstert.2016.04.013
摘要
ObjectiveTo investigate serum microRNAs (miRNAs) in women with endometriosis.DesignCase-control study.SettingUniversity hospital.Patient(s)Women with (n = 24) and without (n = 24) endometriosis.Intervention(s)Serum samples were obtained from surgically diagnosed subjects.Main Outcome Measure(s)miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs.Result(s)miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150–5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model.Conclusion(s)We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance. To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs. miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150–5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model. We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.
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