细胞生物学
细胞周期
胚胎干细胞
雷克斯1
重编程
细胞效价
生物
同源盒蛋白纳米
干细胞
染色质
诱导多能干细胞
细胞
遗传学
基因
作者
Jihoon Shin,Tae Wan Kim,Hyunsoo Kim,Hye-Ji Kim,Min Young Suh,Sang-Ho Lee,Han Teo Lee,Sojung Kwak,Sang Eun Lee,Jong Hyuk Lee,Hyonchol Jang,Eun Jung Cho,Hong Duk Youn
出处
期刊:eLife
[eLife Sciences Publications, Ltd.]
日期:2016-02-15
卷期号:5
被引量:42
摘要
Pluripotency transcription programs by core transcription factors (CTFs) might be reset during M/G1 transition to maintain the pluripotency of embryonic stem cells (ESCs). However, little is known about how CTFs are governed during cell cycle progression. Here, we demonstrate that the regulation of Oct4 by Aurora kinase b (Aurkb)/protein phosphatase 1 (PP1) during the cell cycle is important for resetting Oct4 to pluripotency and cell cycle genes in determining the identity of ESCs. Aurkb phosphorylates Oct4(S229) during G2/M phase, leading to the dissociation of Oct4 from chromatin, whereas PP1 binds Oct4 and dephosphorylates Oct4(S229) during M/G1 transition, which resets Oct4-driven transcription for pluripotency and the cell cycle. Aurkb phosphor-mimetic and PP1 binding-deficient mutations in Oct4 alter the cell cycle, effect the loss of pluripotency in ESCs, and decrease the efficiency of somatic cell reprogramming. Our findings provide evidence that the cell cycle is linked directly to pluripotency programs in ESCs.
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