Dual mode of Triphala in the reversal of cognition through gut restoration in antibiotic mediated prolonged dysbiosis condition in 5XFAD mice

The emerging role of gut-brain axis decoding recently created a new hope for investigating the biological and physiological basis of neurodegenerative disorders and other neurological problems. In this context, we used the bidirectional polyphenols-rich Triphala against the antibiotics cocktail-treated 5XFAD mice to decode the gut-brain axis. After 60 days of orally given Triphala and Antibiotics, the treated group showed significant improvement in the cognitive parameters in the behavioral study Morris water and Y maze. The Triphala treated group showed the neurogenesis, reduced level of amyloid beta in serum, and amyloid precursor protein, mRNA expression in the brain of mice. The serum level and mRNA expression of anti-inflammatory and antioxidant activity were also studied. Simultaneously, the Triphala-treated group showed improved gut transition time and increased butyrate levels in feces. The 16 s rRNA analysis of the V3-V4 region of feces DNA showed more presence of disease-modifying bacteria like Bacteriodetes and Verrucomicrobiota by the percentage of 31 and 23%. The reduction level in percentage abundance of Cyanobacteria indicated the effect of Triphala against AD. The availabilities of these bacteria, and the reversal of cognitive parameters in the AD mice, showed the promising effect of Triphala for treating neurodegenerative disorders.