Effect of microbially induced carbonate precipitation (MICP) on the early strength enhancement and micromechanical properties in fly ash blended cement

Microbially induced carbonate precipitation (MICP), an emerging technique recently, has been investigated in extensive studies for its ability to improve or enhance the properties of materials. This paper mainly explored the effect of MICP on the early strength and hydration products in fly ash blended cement paste (FABC) under different MICP treatment measures. Firstly, the influence of different environment on the growth of microorganism and three calcium source (CaCl2, Ca(CH3COO)2 and Ca(NO3)2) on the production of calcium carbonate precipitation were investigated. The experimental results presented that the efficiency of CaCO3 precipitation in the Ca(NO3)2 sample by adding bacterial solution is higher than other groups. Compared to untreated specimens, the mechanical properties of FABC paste are adversely affected by direct mixing with bacterial solution (MICP1) due to the fact that the hydration reactions of C3S and Ca(OH)2(CH) in FABC system were negatively affected as indicated by the hydration heat measurement. In contrast, the early performance of drying-immersing sample in bacterial solution (MICP2) was improved, with a maximum efficacy of 52.03%. The hydration process was facilitated in FABC paste treated with MICP2 treatment, further leading to increased formation of CH, C-S-H and AFt. Furthermore, Nanoindentation showed the HD C-S-H gel and CH were the dominant phases in MICP2 samples, and the matrix became denser with more hydration products, which also confirmed the increase in macroscopic compressive strength. The findings of this work can provide a guidance for the application of FA based on MICP method for enhancing the early strength in cementitious materials.