子宫腺肌病
Wnt信号通路
生物
化生
间质细胞
癌症研究
干细胞
病理
细胞生物学
子宫内膜异位症
信号转导
医学
作者
Tao Chen,Yu X. Xu,Xiaocui Xu,Jianzhang Wang,Zhen Qiu,Yayuan Yu,Xiaohong Jiang,Wen Shao,Dandan Bai,Mingzhu Wang,Shuyan Mei,Cheng Tien,Lijun Wu,Shaorong Gao,Xuan Che
标识
DOI:10.1093/procel/pwae012
摘要
Adenomyosis is a poorly understood gynecological disorder lacking effective treatments. Controversy persists regarding "invagination" and "metaplasia" theories. The endometrial-myometrial junction (EMJ) connects the endometrium and myometrium and is important for diagnosing and classifying adenomyosis, but its in-depth study is just beginning. Using single-cell RNA sequencing and spatial profiling, we mapped transcriptional alterations across eutopic endometrium, lesions, and EMJ. Within lesions, we identified unique epithelial (LGR5+) and invasive stromal (PKIB+) subpopulations, along with WFDC1+ progenitor cells, supporting a complex interplay between "invagination" and "metaplasia" theories of pathogenesis. Further, we observed endothelial cell heterogeneity and abnormal angiogenic signaling involving VEGF and ANGPT pathways. Cell-cell communication differed markedly between ectopic and eutopic endometrium, with aberrant signaling in lesions involving PTN, TWEAK, and WNT cascades. This study reveals unique stem cell-like and invasive cell subpopulations within adenomyosis lesions identified, dysfunctional signaling, and EMJ abnormalities critical to developing precise diagnostic and therapeutic strategies.
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