Abstract: With the continuous development of material science, many new biomaterials have emerged. Peptides have a strong supramolecular self-assembly ability and can form hydrogels through a self-assembly process. These self-assembled peptide hydrogels have the advantages of excellent biocompatibility, tunability, and degradability, and are suitable for biomedical fields. This paper reviews the mechanisms and characteristics of peptide gel formation, outlines the various factors affecting peptide gelation, and the applications of peptide hydrogels in drug delivery, tissue engineering, and wound healing. Finally, challenges encountered in self-assembled peptide gels and prospects for their application are highlighted.