抗氧化剂
化学
DPPH
抗坏血酸
多糖
羟基自由基
过氧化氢
根茎
食品科学
清除
超氧化物
有机化学
传统医学
酶
医学
作者
Ji-Wen Sheng,Yanlong Sun
标识
DOI:10.1016/j.carbpol.2014.03.011
摘要
Different molecular weight polysaccharides were prepared by degradation of polysaccharides extracted from Athyrium multidentatum (Doll.) Ching rhizome (CPA) with hydrogen peroxide and ascorbic acid. Four low molecular polysaccharides derivatives (CPA-1, CPA-2, CPA-3 and CPA-4) were successfully obtained and had their antioxidant activities investigated employing various established in vitro systems. All CPA derivatives showed pronounced antioxidant activity, and had stronger antioxidant ability than CPA in certain tests. CPA-1 exhibited the strongest scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical among all samples, and the IC50 value was 25 μg/mL. CPA-2 possessed the highest scavenging ability against superoxide radical at 200 μg/mL. The scavenging activity of CPA-4 on hydroxyl radical was higher than CPA from 120 to 200 μg/mL. The mechanism on influence the antioxidant activity of CPA and its degraded derivatives was indicated.
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