三氯生
纤维素
纳米载体
纳米纤维素
细菌纤维素
抗菌活性
化学
大肠杆菌
组合化学
生物膜
药物输送
细菌
有机化学
生物化学
生物
医学
病理
基因
遗传学
作者
Aiswarya Thattaru Thodikayil,Ajay Yadav,P. Hariprasad,Sampa Saha
标识
DOI:10.1016/j.ijbiomac.2023.127604
摘要
Designing a suitable, cost-effective nanocarrier with an ability to capture and deliver antibiotics for restricting microbial spread remains an unmet need. A simple two-stepped strategy involving citric acid-induced hydrolysis of cellulose pulp (NFC) followed by TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) mediated oxidation to obtain carboxylated nano fibrillated cellulose (TNFC-5) with high carboxyl content (1.12 mmol/g) has been explored. TNFC-5 so obtained was able to capture remarkable extent of antibiotics (drug loading (DL) > 40 % and entrapment efficiency (EE) >80 %) irrespective of their hydrophilicity as in, triclosan (hydrophobic) and ampicillin sodium (hydrophilic). In silico molecular docking study revealed the excess carboxyl content in nanocellulose imparted the strongest binding affinity to antibiotics via H-bonding. A slower and sustained release of triclosan was observed for TNFC-5 than that of NFC, reiterating the enhanced binding efficiency of the drugs with TNFC-5. Well-dispersed triclosan loaded TNFC-5 displayed sustained antibacterial activity against Escherichia coli and Staphylococcus aureus up to one week. Thus, TNFC-5 has been demonstrated as a green, cheap, and eco-friendly alternative to the other biodegradable nanocarriers for carrying antibiotics with high DL and EE, thereby reducing the wastage of expensive drugs while ensuring a sustained antibacterial effect. Our study established that the drug loaded nanofibers (TNFC-5) might act as a promising candidate to penetrate through biofilm for treating serious bacterial infections by retarding their growth and eventually eradicating bacterial colonies.
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