Exploring Electrochemistry: A Hydrogen Peroxide Sensor Based on a Screen-Printed Carbon Electrode Modified with Prussian Blue

There is an increasing need for fundamental electrochemistry concepts to be taught in the undergraduate curriculum, given the broad applicability of electrochemical technologies in addressing a wide range of global issues from critical energy shortages to real-time medical diagnostics. However, many electrochemical concepts are often taught in disparate laboratory experiments, spread out through the curriculum, which can be intimidating to students (and instructors). This experiment, which has been tested and optimized in the undergraduate classroom over multiple semesters, covers a wide range of electrochemistry topics in realizing the construction of a hydrogen peroxide (H2O2) sensor that is based on Prussian blue electrochemistry. The experiment introduces the fundamentals of cyclic voltammetry by prompting students to distinguish faradaic and capacitive components of voltammograms and to investigate their relationship with scan rate as per electrochemical theory. Students also evaluate electrocatalysis through electrodeposition of a thin film of Prussian blue on the sensor surface and the effects of this modification on electron transfer and sensor performance. Finally, students combine amperometric measurements with the method of standard additions to determine H2O2 concentrations in an unknown sample. Overall, this experiment offers an integrated and cohesive experience that connects many important electroanalytical concepts that are often taught individually into one 3 h, hands-on laboratory experiment that requires minimal resources.