Ⅰ型胶原
间质细胞
弹性模量
细胞外基质
分子
生物物理学
材料科学
类有机物
癌细胞
化学
动态力学分析
聚合物
癌症
复合材料
生物化学
癌症研究
细胞生物学
有机化学
医学
病理
内科学
生物
作者
Tomoyuki Suezawa,Naoki Sasaki,Yasuhiro Yukawa,Nazgul Assan,Yuta Uetake,Kunishige Onuma,R. F. Kamada,Daisuke Tomioka,Hidehiro Sakurai,Ryohei Katayama,Masahiro Inoue,Michiya Matsusaki
标识
DOI:10.1002/advs.202302637
摘要
Abstract Collagen is the most abundant protein in the human body and one of the main components of stromal tissues in tumors which have a high elastic modulus of over 50 kPa. Although collagen has been widely used as a cell culture scaffold for cancer cells, there have been limitations when attempting to fabricate a tough collagen gel with cells like a cancer stroma. Here, rapid gelation of a collagen solution within a few minutes by transition metal complexation is demonstrated. Type I collagen solution at neutral pH shows rapid gelation with a transparency of 81% and a high modulus of 1,781 kPa by mixing with K 2 PtCl 4 solution within 3 min. Other transition metal ions also show the same rapid gelation, but not basic metal ions. Interestingly, although type I to IV collagen molecules show rapid gelation, other extracellular matrices do not exhibit this phenomenon. Live imaging of colon cancer organoids in 3D culture indicates a collective migration property with modulating high elastic modulus, suggesting activation for metastasis progress. This technology will be useful as a new class of 3D culture for cells and organoids due to its facility for deep‐live observation and mechanical stiffness adjustment.
科研通智能强力驱动
Strongly Powered by AbleSci AI