生物
前脑
后脑
胶质瘤
胚胎干细胞
内含子
癌症研究
细胞生物学
基因
突变体
遗传学
神经科学
中枢神经系统
作者
Kosuke Funato,R. V. Smith,Yuhki Saito,Viviane Tabar
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2021-05-01
卷期号:28 (5): 894-905.e7
被引量:33
标识
DOI:10.1016/j.stem.2021.02.003
摘要
H3.3G34R-mutant gliomas are lethal tumors of the cerebral hemispheres with unknown mechanisms of regional specificity and tumorigenicity. We developed a human embryonic stem cell (hESC)-based model of H3.3G34R-mutant glioma that recapitulates the key features of the tumors with cell-type specificity to forebrain interneuronal progenitors but not hindbrain precursors. We show that H3.3G34R, ATRX, and TP53 mutations cooperatively impact alternative RNA splicing events, particularly suppression of intron retention. This leads to increased expression of components of the Notch pathway, notably NOTCH2NL, a human-specific gene family. We also uncover a parallel mechanism of enhanced NOTCH2NL expression via genomic amplification of its locus in some H3.3G34R-mutant tumors. These findings demonstrate a novel mechanism whereby evolutionary pathways that lead to larger brain size in humans are co-opted to drive tumor growth.
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