农杆菌
卡那霉素
生物
β-葡萄糖醛酸酶
转基因作物
转化(遗传学)
转基因
基因
植物
外植体培养
遗传学
基因表达
体外
作者
Kishchenko Em,Komarnitskiĭ Ik,М. В. Кучук
出处
期刊:PubMed
日期:2005-08-01
卷期号:39 (1): 9-13
被引量:2
摘要
Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants without hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.
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